Rabbit TrueBlot®: Anti-Rabbit IgG HRP (cat. Mouse TrueBlot®: Anti-Mouse Ig Biotin (cat. This TrueBlot Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG) Epitope Tag allows for the detection of FLAG-tagged recombinant protein present in. TrueBlot® reagents are available in several options, from IP Beads alone, to complete IP/Western Blot kits from goat, mouse, rabbit or sheep: Mouse TrueBlot Products determine post-translational protein modifications. This approach enables researchers to: identify the activation status of proteins. TrueBlot schematic experimental workflow (Rockland Immunochemicals – tebu-bio) Immunoprecipitation (IP) is the small-scale affinity purification of antigens using a specific antibody and is one of the most widely used methods for antigen purification and detection. The basic TrueBlot® concept map is depicted below and outlines the major mechanisms by which TrueBlot® can create exceptionally clear results. phosphorylation, or protein-protein interactions. Ideal for studying post-translational protein modifications, e.g.Accurate Target Detection – TrueBlot® preferentially detects native (non-denatured) Ig, ensuring you are only detecting your target protein.Immunoblotting can identify immunoprecipitated proteins more specifically and with higher sensitivity than nonspecific protein stains or autoradiography. Combining the procedures of immunoprecipitation and immunoblotting can help to overcome some of the limitations of each separate procedure. Easy to Use – HRP-conjugated TrueBlot® anti-Ig simply replaces your regular HRP-conjugated secondary antibody. Immunoprecipitation-western blot for proteins of low abundance. When probing immunoprecipitated samples on a western blot, the concentration of primary antibody can be increased resulting in an increase in sensitivity.The TrueBlot® products are available for mouse, rabbit, sheep and goat primary antibodies. IP/Western blots thus obtained are highly specific with an increased sensitivity, less background noise, and enhanced accuracy. TrueBlot® immuno-blotting reagents are easy to use: simply replace your conventional HRP or fluorescent reagent with a TrueBlot® for publication-quality IP & WB data. Thus, only detecting your target protein and providing unparalleled clarity in results and high-quality imaging suitable for reproduction. Indeed, the TrueBlot® secondary antibodies preferentially detect the native disulfide form of your primary antibody and effectively eliminate the “artificial” bands. It eliminates 55 kDa heavy and ~23 kDa light chains from masking your proteins of interest. TrueBlot® products are prominent tools for bypassing these artefacts in IP & WB, whilst increasing the sensitivity of immunoassays for publication-grade WB.
0 kommentar(er)
